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Anti-Cyclin B1 Antibody
Catalog #: 188-10016
Mouse anti-Human/Mouse/Hamster Cyclin B1 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Specificity | Other species not known |
| Host Species | Mouse |
| Accession Number | P14635 |
| Gene Id | 891 |
| Gene Symbols | CCNB1,CCNB |
| Protein Name / Synonyms | G2/mitotic-specific cyclin-B1 |
| Immunogen | Hamster protein was used as the immunogen for this Cyclin B1 antibody. |
| Isotype | Kappa, IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 0.2 mg/ml in 1X PBS (pH 7.4) with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Recommended Applications | FACS: 0.5-1ug/million cells IF: 1-2ug/ml IP: 1-2ug/500ug protein--precipitates active Cyclin B1 complexes |
| Domain / Region / Terminus | Cytoplasmic |
| Research Area | Cell Cycle |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
This antibody recognizes a protein of 55-62kDa, identified as Cyclin B1. In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Cyclin B1-p34cdc2 plays a critical role in G2 to M transition.
Storage
Store the Cyclin B1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.