Anti-CYP2F1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P24903
Gene Id	1572
Gene Symbols	CYP2F1
Protein Name / Synonyms	CYP2F1, C2F1, CYP2F, CYPIIF1, cytochrome P450 2F1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to dehydrogenate 3-methylindole, an endogenous toxin derived from the fermentation of tryptophan, as well as xenobiotic substrates such as naphthalene and ethoxycoumarin. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

36kDa/55kDa

Observed MW

46kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-290 of human CYP2F1 (NP_000765.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CYP2F1 antibody (144-07550) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - CYP2F1 antibody (144-07550)

Immunohistochemistry of paraffin-embedded mouse lung using CYP2F1 antibody (144-07550) at dilution of 1:200 (40x lens).
Immunohistochemistry - CYP2F1 antibody (144-07550)

Expiration:

12 months from the date of shipment when stored properly.

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