Product Description

Specifications

Size	20 µg, 100 µg
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Specificity	Other species not tested.
Host Species	Mouse
Accession Number	P05783
Gene Id	3875
Gene Symbols	KRT18, CYK18, PIG46
Protein Name / Synonyms	Keratin, type I cytoskeletal 18 (Cell proliferation-inducing gene 46 protein) (Cytokeratin-18) (CK-18) (Keratin-18) (K18)
Immunogen	Recombinant full-length human KRT18 protein was used as the immunogen for the Cytokeratin 18 antibody.
Isotype	IgG1
Purity	Protein G affinity chromatography
Concentration (lot specific)	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Recommended Applications	Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 1-2ug/ml Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2)
Domain / Region / Terminus	Cytoplasmic
Research Area	Cancer
Shipping Type	Blue ice
Storage	≤-20°C

Description

This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.