Anti-Cytokeratin 2 (internal) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	3849
Protein Name / Synonyms	Cytokeratin 2, Internal
Concentration (lot specific)	0.67 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

CK-2e antibody, K2e antibody, KRT2A antibody, KRT2E antibody, KRTE antibody, MGC116967 antibody, MGC116968 antibody, KRT2 antibody, epidermal ichthyosis bullosa of Siemens antibody, epithelial keratin-2e antibody, keratin-2 epidermis antibody, cytokeratin-2e antibody, "keratin, type II cytoskeletal 2 epidermal antibody", keratin-2e antibody, type-II keratin Kb2 antibody, keratin 2 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

65 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 143 and 529 of Cytokeratin 2 (Uniprot ID# P35908)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 2(107-10350) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using KRT2(107-10350) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: A431

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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