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Anti-Cytokeratin 5 + 8 Antibody, Sodium Azide Free

Catalog #: 188-10852-SAF

Mouse anti-Human/Pig/Mouse/Rat/Hamster/Cow/Dog/Sheep/Ferret Cytokeratin 5 + 8 Antibody [Sodium Azide Free]

Product Description

Specifications

Size100 µg
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesMouse
Accession Number
P13647,P05787
Gene Id
3856|3852
Gene Symbols
KRT8,KRT5,CYK8
Protein Name / Synonyms
Keratin, type II cytoskeletal 5 (58 kDa cytokeratin) (Cytokeratin-5) (CK-5) (Keratin-5) (K5) (Type-II keratin Kb5), Keratin, type II cytoskeletal 8 (Cytokeratin-8) (CK-8) (Keratin-8) (K8) (Type-II keratin Kb8)
Immunogen
A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5 + 8 antibody.
IsotypeKappa, IgG1
Purity
Protein G affinity chromatography
Concentration (lot specific)
1 mg/ml in 1X PBS, BSA free, sodium azide free
Recommended Applications
Flow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 0.5-1ug/ml
Western blot: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Domain / Region / TerminusCytoplasmic
Shipping TypeBlue ice
Storage≤-20°C

Description

Description

It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Storage

Store the Cytokeratin 5/8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.