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Anti-Cytokeratin 8 + 18 Antibody Cocktail, Sodium Azide Free
Catalog #: 188-10211-SAF
Mouse anti-Human Cytokeratin 8 + 18 Antibody Cocktail [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | Other species not known |
| Host Species | Mouse |
| Accession Number | P05787 |
| Gene Id | 3856 |
| Gene Symbols | KRT8,CYK8 |
| Protein Name / Synonyms | Keratin, type II cytoskeletal 8 (Cytokeratin-8) (CK-8) (Keratin-8) (K8) (Type-II keratin Kb8) |
| Immunogen | A keratin preparation from a human carcinoma (K8.8) and PMC-42 human breast carcinoma cells (DC10) were used as the immunogen for this Cytokeratin 8 + 18 antibody cocktail. |
| Isotype | Kappa, IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS (pH 7.4), BSA free, sodium azide free |
| Recommended Applications | FACS: 0.5-1ug/million cells IF: 1-2ug/ml Western Blotting: 0.5-1.0ug/ml IHC (FFPE): 0.5-1.0ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Cytoplasmic |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Cytokeratins are a group of proteins expressed in pairs in keratinized and non-keratinized epithelial tissue. Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). The pair are normally seen in simple but not stratified epithelial cells. This antibody cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. Immunohistochemical staining with this antibody cocktail is indistinguishable from that obtained with monoclonal antibody 5D3.
Storage
Store the Cytokeratin 8/18 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.