Anti-DAO Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P14920
Gene Id	1610
Gene Symbols	DAO
Protein Name / Synonyms	DAO, DAAO, DAMOX, OXDA, D-amino-acid oxidase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

39kDa

Observed MW

39kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-347 of human DAO (NP_001908.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of mouse kidney, using DAO antibody (144-05309) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - DAO antibody (144-05309)

Immunofluorescence analysis of MCF-7 cells using DAO antibody (144-05309). Blue: DAPI for nuclear staining.
Immunofluorescence - DAO antibody (144-05309)

Expiration:

12 months from the date of shipment when stored properly.

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