Anti-DAPK1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P53355
Gene Id	1612
Gene Symbols	DAPK1
Protein Name / Synonyms	DAPK1, DAPK, ROCO3, death-associated protein kinase 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cancer, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW

36kDa/152kDa/160kDa/161kDa

Observed MW

160kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1141-1430 of human DAPK1 (NP_004929.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using DAPK1 Antibody (144-05741) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Western blot - DAPK1 Polyclonal Antibody (144-05741)

Expiration:

12 months from the date of shipment when stored properly.

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