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Anti-DARS2 Antibody
Catalog #: 144-07813
DARS2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q6PI48 |
| Gene Id | 55157 |
| Gene Symbols | DARS2 |
| Protein Name / Synonyms | DARS2, ASPRS, LBSL, MT-ASPRS, aspartate--tRNA ligase, mitochondrial |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL).
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
73kDa
Observed MW
74kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 406-645 of human DARS2 (NP_060592.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DARS2 antibody (144-07813) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver injury using DARS2 antibody (144-07813) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DARS2 antibody (144-07813) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using DARS2 antibody (144-07813) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.