Anti-DAZ3 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9NR90
Gene Id	57054
Gene Symbols	DAZ3
Protein Name / Synonyms	DAZ3 (C-term)
Clonality	Polyclonal
Preparation	This DAZ3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from the C-terminal region of human DAZ3.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains one copy of the 10.8 kb repeat.

Specificity

Other Names

DAZ3; Deleted in azoospermia protein 3

NCBI Accession #

NP_065097.2

Other Accession #

Q86SG3; Q13117; Q9NQZ3; NP_065097.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

54989

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	DAZ3 Antibody (C-term) (Cat# 102-11494) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the DAZ3 antibody detected the DAZ3 protein (arrow).
	DAZ3 Antibody (C-term) (Cat# 102-11494)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DAZ3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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