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Anti-DBH Antibody
Catalog #: 144-02711
DBH Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P09172 |
| Gene Id | 1621 |
| Gene Symbols | DBH |
| Protein Name / Synonyms | DBH, DBM, dopamine beta-hydroxylase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Neuroscience |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
69kDa
Observed MW
79kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human DBH (NP_000778.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of mouse brain, using DBH antibody (144-02711) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using DBH antibody (144-02711) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using DBH antibody (144-02711) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.