Anti-DCAF7 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P61962
Gene Id	10238
Gene Symbols	DCAF7
Protein Name / Synonyms	DCAF7, AN11, HAN11, SWAN-1, WDR68, DDB1 and CUL4 associated factor 7
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein with multiple WD40 repeats which facilitate protein-protein interactions and thereby enable the assembly of multiprotein complexes. This protein has been shown to function as a scaffold protein for protein complexes involved in kinase signaling. This highly conserved gene is present in eukaryotic plants, fungi, and animals. The ortholog of this gene was first identified in plants as a key regulator of anthocyanin biosynthesis and flower pigmentation. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IF 1:10 - 1:100

Format

Calculated MW

16kDa/38kDa

Observed MW

39kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human DCAF7 (NP_005819.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of 293T cells, using DCAF7 antibody (144-06787) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - DCAF7 Polyclonal Antibody (144-06787)

Immunofluorescence analysis of MCF7 cells using DCAF7 antibody (144-06787). Blue: DAPI for nuclear staining.
Immunofluorescence - DCAF7 Polyclonal Antibody (144-06787)

Expiration:

12 months from the date of shipment when stored properly.

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