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Anti-DCK Antibody
Catalog #: 144-01794
DCK Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P27707 |
| Gene Id | 1633 |
| Gene Symbols | DCK |
| Protein Name / Synonyms | DCK, deoxycytidine kinase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
30kDa
Observed MW
31kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DCK (NP_000779.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DCK Antibody (144-01794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat kidney using DCK antibody (144-01794) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using DCK antibody (144-01794) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using DCK antibody (144-01794) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using DCK antibody (144-01794) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using DCK antibody (144-01794). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.