3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-DCP2 Antibody
Catalog #: 144-08282
DCP2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q8IU60 |
| Gene Id | 167227 |
| Gene Symbols | DCP2 |
| Protein Name / Synonyms | DCP2, NUDT20, decapping mRNA 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
44kDa/48kDa
Observed MW
50kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human DCP2 (NP_001229306.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DCP2 antibody (144-08282) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using DCP2 antibody (144-08282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using DCP2 antibody (144-08282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using DCP2 antibody (144-08282) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DCP2 antibody (144-08282) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.