Anti-DCTN2 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q13561
Gene Id	10540
Gene Symbols	DCTN2
Protein Name / Synonyms	DCTN2 (Center)
Clonality	Polyclonal
Preparation	This DCTN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-219 amino acids from the Central region of human DCTN2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

DCTN2; DCTN50; Dynactin subunit 2; 50 kDa dynein-associated polypeptide; Dynactin complex 50 kDa subunit; p50 dynamitin

NCBI Accession #

NP_001248341.1;NP_001248342.1;NP_006391.1

Other Accession #

Q6AYH5; Q99KJ8; Q3ZCF0; NP_006391.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

44231

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of lysate from SH-SY5Y cell line, using DCTN2 Antibody (Center)(Cat# 102-11782). 102-11782 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
	DCTN2 Antibody (Center) (Cat# 102-11782)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DCTN2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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