DDB1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16531 |
| Gene Id | 1642 |
| Gene Symbols | DDB1 |
| Protein Name / Synonyms | DDB1, DDBA, UV-DDB1, XAP1, XPCE, XPE, XPE-BF, DNA damage-binding protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
50kDa/126kDa
Observed MW
127kDa
Immunogen
A synthetic peptide of human DDB1
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DDB1 Antibody (144-02896) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human uterine cancer using DDB1 Antibody (144-02896) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using DDB1 Antibody (144-02896) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using DDB1 Antibody (144-02896) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using DDB1 Antibody (144-02896) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using DDB1 Antibody (144-02896) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using DDB1 Antibody (144-02896) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using DDB1 Antibody (144-02896) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DDB1 Antibody (144-02896) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using DDB1 Antibody (144-02896) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human brain cancer using DDB1 Antibody (144-02896) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using DDB1 Antibody (144-02896) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using DDB1 Antibody (144-02896) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using DDB1 Antibody (144-02896) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using DDB1 Antibody (144-02896) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using DDB1 Antibody (144-02896) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using DDB1 Antibody (144-02896) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DDB1 Antibody (144-02896) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.