Anti-DDIT3 (C-term A135) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Mouse
Host Species	Rabbit
Accession Number	P35638
Gene Id	1649
Gene Symbols	DDIT3
Protein Name / Synonyms	DDIT3 (C-term A135)
Clonality	Polyclonal
Preparation	This DDIT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-149 amino acids from the C-terminal region of human DDIT3.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	FC, Western Blotting, IF
Research Area	Cancer, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Specificity

Predicted Reactivity

Bovine, Hamster

Other Names

DDIT3; CHOP; CHOP10; GADD153; DNA damage-inducible transcript 3 protein; C/EBP-homologous protein; C/EBP-homologous protein 10; Growth arrest and DNA damage-inducible protein GADD153

NCBI Accession #

NP_001181982.1;NP_001181983.1;NP_001181984.1;NP_001181986.1;NP_004074.2

Other Accession #

P35639; P14607; Q0IIB6; NP_004074

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

19175

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; FC: 1:10-50

	Fluorescent image of Hela cell stained with DDIT3 Antibody (C-term A135)(Cat# 102-11328/SA101207AA).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DDIT3 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). DDIT3 immunoreactivity is localized to Cytoplasm significantly.
	DDIT3 Antibody (C-term A135) (Cat# 102-11328) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the DDIT3 antibody detected the DDIT3 protein (arrow).
	DDIT3 Antibody (C-term A135) (Cat# 102-11328) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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