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Anti-DDOST Antibody
Catalog #: 144-09056
DDOST Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P39656 |
| Gene Id | 1650 |
| Gene Symbols | DDOST |
| Protein Name / Synonyms | DDOST, AGER1, CDG1R, GATD6, OKSWcl45, OST, OST48, WBP1, dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
46kDa/49kDa/50kDa
Observed MW
51kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human DDOST (NP_005207.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DDOST antibody (144-09056) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Expiration:
12 months from the date of shipment when stored properly.