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Anti-DDX20 Antibody
Catalog #: 144-05817
DDX20 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9UHI6 |
| Gene Id | 11218 |
| Gene Symbols | DDX20 |
| Protein Name / Synonyms | DDX20, DP103, GEMIN3, DEAD-box helicase 20 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
IHC 1:50 - 1:200
IF 1:10 - 1:100
Format
Calculated MW
14kDa/92kDa
Observed MW
110kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human DDX20 (NP_009135.4).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DDX20 antibody (144-05817) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using DDX20 Antibody (144-05817) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human amygdalitis using DDX20 Antibody (144-05817) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using DDX20 Antibody (144-05817) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using DDX20 antibody (144-05817). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.