RayBiotech

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Anti-DDX39A Antibody

Catalog #: 144-07955

DDX39A Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
O00148
Gene Id
10212
Gene Symbols
DDX39A
Protein Name / Synonyms
DDX39A, BAT1, BAT1L, DDX39, DDXL, URH49, DExD-box helicase 39A
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the DEAD box protein family. These proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is thought to play a role in the prognosis of patients with gastrointestinal stromal tumors. A pseudogene of this gene is present on chromosome 13. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW
30kDa/36kDa/49kDa
Observed MW
49kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 178-427 of human DDX39A (NP_005795.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using DDX39A antibody (144-07955) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Western blot - DDX39A antibody (144-07955)

Immunohistochemistry of paraffin-embedded mouse kidney using DDX39A antibody (144-07955) at dilution of 1:100 (40x lens).
Immunohistochemistry - DDX39A antibody (144-07955)

Immunohistochemistry of paraffin-embedded rat brain using DDX39A antibody (144-07955) at dilution of 1:100 (40x lens).
Immunohistochemistry - DDX39A antibody (144-07955)

Immunohistochemistry of paraffin-embedded human stomach using DDX39A antibody (144-07955) at dilution of 1:100 (40x lens).
Immunohistochemistry - DDX39A antibody (144-07955)

Expiration:
12 months from the date of shipment when stored properly.