DDX5 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P17844 |
| Gene Id | 1655 |
| Gene Symbols | DDX5 |
| Protein Name / Synonyms | DDX5, G17P1, HLR1, HUMP68, p68, DEAD-box helicase 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
60kDa/69kDa
Observed MW
69kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-614 of human DDX5 (NP_004387.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DDX5 antibody (144-60268) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat lung using DDX5 antibody (144-60268) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DDX5 antibody (144-60268) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using DDX5 antibody (144-60268) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using DDX5 antibody (144-60268) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using DDX5 antibody (144-60268) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using DDX5 antibody (144-60268) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using DDX5 antibody (144-60268). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.