Anti-DDX58 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Accession Number	O95786
Gene Id	23586
Gene Symbols	DDX58
Protein Name / Synonyms	DDX58, RIG-I, RIGI, RLR-1, SGMRT2, DExD/H-box helicase 58
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF, IP
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
IP 1:20 - 1:100

Format

Calculated MW

101kDa/106kDa

Observed MW

109kDa

Immunogen

A synthetic peptide of human DDX58

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using DDX58 antibody (144-00550) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Western blot - DDX58 Polyclonal Antibody (144-00550)

Immunohistochemistry of paraffin-embedded human prostate using DDX58 antibody (144-00550) at dilution of 1:100 (40x lens).
Immunohistochemistry - DDX58 Polyclonal Antibody (144-00550)

Immunofluorescence analysis of HeLa cells using DDX58 antibody (144-00550). Blue: DAPI for nuclear staining.
Immunofluorescence - DDX58 Polyclonal Antibody (144-00550)

Expiration:

12 months from the date of shipment when stored properly.

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