DFNA5 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O60443 |
| Gene Id | 1687 |
| Gene Symbols | DFNA5 |
| Protein Name / Synonyms | DFNA5, ICERE-1, non-syndromic hearing impairment protein 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Concentration (lot specific) | 2.61 mg/ml |
| Buffers | PBS with 0.05% proclin300, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
10kDa/36kDa/54kDa
Observed MW
50kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DFNA5 (NP_004394.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DFNA5 Antibody (144-07432) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human liver injury using DFNA5 antibody (144-07432) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DFNA5 antibody (144-07432) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using DFNA5 antibody (144-07432) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DFNA5 antibody (144-07432) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
