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Anti-DGAT1 Antibody
Catalog #: 144-06857
DGAT1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O75907 |
| Gene Id | 8694 |
| Gene Symbols | DGAT1 |
| Protein Name / Synonyms | DGAT1, ARAT, ARGP1, DGAT, DIAR7, diacylglycerol O-acyltransferase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
55kDa
Observed MW
54kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human DGAT1 (NP_036211.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DGAT1 antibody (144-06857) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat pancreas using DGAT1 antibody (144-06857) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using DGAT1 antibody (144-06857) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using DGAT1 antibody (144-06857) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using DGAT1 antibody (144-06857) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.