3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-DHO Antibody
Catalog #: 130-10088
Rabbit Anti-Human DHO Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | O75874 |
| Gene Id | 3417 |
| Gene Symbols | IDH1 PICD |
| Clonality | Polyclonal |
| Immunogen | Immunogen was recombinant protein from human isocitrate dehydrogenases. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1:320, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Cancer |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Isocitrate dehydrogenases appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.
Specificity
The antibody binds to human isocitrate dehydrogenases. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat isocitrate dehydrogenases was not tested.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
Related Products
Rabbit Anti-Alcohol Dehydrogenase (cat# MD-14-0981) Mouse Anti-Alcohol Dehydrogenase (cat#MD-15-0109)
References
- Bleeker FE, et al.. (2009) IDH1 mutations at residue p.R132 (IDH1(R132)) occur frequently in high-grade gliomas but not in other solid tumors. Hum Mutat. 30(1):7-11.
- Murugan AK, et al.. (2010) Identification and functional characterization of isocitrate dehydrogenase 1 (IDH1) mutations in thyroid cancer. Biochem Biophys Res Commun. 393(3):555-9.
Expiration:
12 months from the date of shipment when stored properly.