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Anti-DHRS2 Antibody
Catalog #: 144-06446
DHRS2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13268 |
| Gene Id | 10202 |
| Gene Symbols | DHRS2 |
| Protein Name / Synonyms | DHRS2, HEP27, SDR25C1, dehydrogenase/reductase 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family, which has over 46,000 members. Members of this family are enzymes that metabolize many different compounds, such as steroid hormones, prostaglandins, retinoids, lipids and xenobiotics. Alternative promoter use and alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
IHC 1:50 - 1:200
IF 1:10 - 1:100
Format
Calculated MW
29kDa/31kDa
Observed MW
33kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DHRS2 (NP_878912.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DHRS2 antibody (144-06446) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human breast cancer using DHRS2 antibody (144-06446) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using DHRS2 antibody (144-06446) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DHRS2 antibody (144-06446) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using DHRS2 antibody (144-06446). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.