Anti-DHRS9 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9BPW9
Gene Id	10170
Gene Symbols	DHRS9
Protein Name / Synonyms	DHRS9, 3-alpha-HSD, 3ALPHA-HSD, RDH-E2, RDH-TBE, RDH15, RDHL, RDHTBE, RETSDR8, SDR9C4, dehydrogenase/reductase 9
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. This protein demonstrates oxidoreductase activity toward hydroxysteroids and is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100

Format

Calculated MW

18kDa/30kDa/35kDa/41kDa

Observed MW

33kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 18-319 of human DHRS9 (NP_001135743.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using DHRS9 antibody (144-06324) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - DHRS9 Polyclonal Antibody (144-06324)

Immunohistochemistry of paraffin-embedded rat brain using DHRS9 Antibody (144-06324) at dilution of 1:100 (40x lens).
Immunohistochemistry - DHRS9 Polyclonal Antibody (144-06324)

Immunohistochemistry of paraffin-embedded mouse brain using DHRS9 Antibody (144-06324) at dilution of 1:100 (40x lens).
Immunohistochemistry - DHRS9 Polyclonal Antibody (144-06324)

Immunofluorescence analysis of U2OS cells using DHRS9 antibody (144-06324). Blue: DAPI for nuclear staining.
Immunofluorescence - DHRS9 Polyclonal Antibody (144-06324)

Expiration:

12 months from the date of shipment when stored properly.

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