DiMethyl-Histone H3-K27 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16695 |
| Gene Id | 8290 |
| Gene Symbols | H3K27ME2 |
| Protein Name / Synonyms | HIST3H3, H3.4, H3/g, H3FT, H3t, histone H3.1t |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP, ChIP, ChIPseq |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
CHIPseq 1:20 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
CHIPseq 1:20 - 1:100
Format
Calculated MW
15kDa
Observed MW
18kDa
Immunogen
A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
Modification
DiMethyl K27
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K27 antibody (144-02362).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K27 antibody (144-02362) at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat lung using DiMethyl-Histone H3-K27 antibody (144-02362) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using DiMethyl-Histone H3-K27 antibody (144-02362) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DiMethyl-Histone H3-K27 antibody (144-02362) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using DiMethyl-Histone H3-K27 antibody (144-02362) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse heart using DiMethyl-Histone H3-K27 antibody (144-02362) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using DiMethyl-Histone H3-K27 antibody (144-02362) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse lung using DiMethyl-Histone H3-K27 antibody (144-02362) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K27 antibody (144-02362) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat kidney using DiMethyl-Histone H3-K27 antibody (144-02362) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat liver using DiMethyl-Histone H3-K27 antibody (144-02362) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K27 antibody (144-02362). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis extracts of 293 cell line, using DiMethyl-Histone H3-K27 antibody (144-02362) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Expiration:
12 months from the date of shipment when stored properly.
