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Anti-DLD Antibody
Catalog #: 144-05403
DLD Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P09622 |
| Gene Id | 1738 |
| Gene Symbols | DLD |
| Protein Name / Synonyms | DLD, DLDD, DLDH, E3, GCSL, LAD, PHE3, dihydrolipoamide dehydrogenase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
43kDa/49kDa/54kDa
Observed MW
55kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 36-300 of human DLD (NP_000099.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DLD antibody (144-05403) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human thyroid cancer using DLD Antibody (144-05403) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using DLD Antibody (144-05403) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human endometrial cancer using DLD Antibody (144-05403) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.