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Anti-DLG1 Antibody
Catalog #: 144-08542
DLG1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q12959 |
| Gene Id | 1739 |
| Gene Symbols | DLG1 |
| Protein Name / Synonyms | DLG1, DLGH1, SAP-97, SAP97, dJ1061C18.1.1, hdlg, disks large homolog 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
87-103kDa
Observed MW
100kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human DLG1 (NP_004078.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DLG1 antibody (144-08542) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using DLG1 antibody (144-08542) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using DLG1 antibody (144-08542) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using DLG1 antibody (144-08542) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.