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Anti-DLK1 Antibody
Catalog #: 144-06578
DLK1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P80370 |
| Gene Id | 8788 |
| Gene Symbols | DLK1 |
| Protein Name / Synonyms | DLK1, DLK, DLK-1, Delta1, FA1, PREF1, Pref-1, ZOG, pG2, protein delta homolog 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a transmembrane protein that contains multiple epidermal growth factor repeats that functions as a regulator of cell growth. The encoded protein is involved in the differentiation of several cell types including adipocytes. This gene is located in a region of chromosome 14 frequently showing unparental disomy, and is imprinted and expressed from the paternal allele. A single nucleotide variant in this gene is associated with child and adolescent obesity and shows polar overdominance, where heterozygotes carrying an active paternal allele express the phenotype, while mutant homozygotes are normal.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
33kDa/41kDa
Observed MW
45kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 83-383 of human DLK1 (NP_003827.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DLK1 antibody (144-06578) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded mouse stomach using DLK1 antibody (144-06578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using DLK1 antibody (144-06578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using DLK1 antibody (144-06578) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.