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Anti-DMAP1 Antibody
Catalog #: 144-02324
DMAP1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9NPF5 |
| Gene Id | 55929 |
| Gene Symbols | DMAP1 |
| Protein Name / Synonyms | DMAP1, DNMAP1, DNMTAP1, EAF2, MEAF2, SWC4, DNA methyltransferase 1-associated protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described.
Dilutions
WB 1:1000 - 1:4000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
52kDa
Observed MW
60kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human DMAP1 (NP_061973.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DMAP1 antibody (144-02324) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using DMAP1 antibody (144-02324) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DMAP1 antibody (144-02324) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using DMAP1 antibody (144-02324) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DMAP1 antibody (144-02324) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.