Anti-DMRTA2 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Zebrafish
Host Species	Rabbit
Accession Number	Q96SC8
Gene Id	63950
Gene Symbols	DMRTA2
Protein Name / Synonyms	DMRTA2 (C-term)
Clonality	Polyclonal
Preparation	This DMRTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

DMRTA2 may be involved in sexual development.

Specificity

Predicted Reactivity

Bovine

Other Names

DMRTA2; DMRT5; Doublesex- and mab-3-related transcription factor A2; Doublesex- and mab-3-related transcription factor 5

NCBI Accession #

NP_115486.1

Other Accession #

A2A9A2; A6QQ94; NP_115486.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

53356

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

DMRTA2 Antibody (C-term) (Cat# 102-14496) western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).This demonstrates the DMRTA2 antibody detected the DMRTA2 protein (arrow).

Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody (C-term)(Cat# 102-14496).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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