DNAJB1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P25685 |
| Gene Id | 3337 |
| Gene Symbols | DNAJB1 |
| Protein Name / Synonyms | DNAJB1, HSPF1, Hdj1, Hsp40, RSPH16B, Sis1, dnaJ homolog subfamily B member 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
27kDa/38kDa
Observed MW
39kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human DNAJB1 (NP_006136.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DNAJB1 antibody (144-05504) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.![Western blot - [KO Validated] DNAJB1 Polyclonal Antibody (144-05504)](https://cdn.sanity.io/images/g51potcz/production/1521538a7ae76d31daf15a7302ea885c74dc57db-464x350.jpg)
Western blot analysis of extracts of various cells, using DNAJB1 antibody (144-05504) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.![Western blot - [KO Validated] DNAJB1 Polyclonal Antibody (144-05504)](https://cdn.sanity.io/images/g51potcz/production/1c717337b0a82df15097e9f2ebc9875b1ae4eb0e-366x550.jpg)
Western blot analysis of extracts from normal (control) and DNAJB1 knockout (KO) 293T cells, using DNAJB1 antibody (144-05504) at 1:1000 dilution.![Western blot - [KO Validated] DNAJB1 Polyclonal Antibody (144-05504)](https://www.raybiotech.com/files/images/Antibodies/144/144-05504/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.