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Anti-DNAJB6 Antibody
Catalog #: 144-07052
DNAJB6 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O75190 |
| Gene Id | 10049 |
| Gene Symbols | DNAJB6 |
| Protein Name / Synonyms | DNAJB6, DJ4, DnaJ, HHDJ1, HSJ-2, HSJ2, LGMD1D, LGMD1E, MRJ, MSJ-1, dnaJ homolog subfamily B member 6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
26kDa/30kDa/35kDa/36kDa
Observed MW
40kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 177-326 of human DNAJB6 (NP_490647.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DNAJB6 antibody (144-07052) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using DNAJB6 antibody (144-07052) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using DNAJB6 antibody (144-07052) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using DNAJB6 antibody (144-07052) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using DNAJB6 antibody (144-07052) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse ileum using DNAJB6 antibody (144-07052) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DNAJB6 antibody (144-07052). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using DNAJB6 antibody (144-07052) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.