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Anti-DNAL1 Antibody
Catalog #: 144-08267
DNAL1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q4LDG9 |
| Gene Id | 83544 |
| Gene Symbols | DNAL1 |
| Protein Name / Synonyms | DNAL1, C14orf168, CILD16, dynein axonemal light chain 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes an axonemal dynein light chain which functions as a component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. The encoded protein is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. Alternate splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
8kDa/17kDa/21kDa
Observed MW
22kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 41-190 of human DNAL1 (NP_113615.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of mouse testis, using DNAL1 antibody (144-08267) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using DNAL1 antibody (144-08267) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using DNAL1 antibody (144-08267) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DNAL1 antibody (144-08267) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DNAL1 antibody (144-08267) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.