Anti-DNM1L Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O00429
Gene Id	10059
Gene Symbols	DNM1L
Protein Name / Synonyms	DNM1L, DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV, dynamin 1 like
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

60kDa/78kDa/79kDa/80kDa/81kDa/82kDa/83kDa

Observed MW

82kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 411-710 of human DNM1L (NP_036193.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using DNM1L antibody (144-02586) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Western blot - DNM1L Polyclonal Antibody (144-02586)

Western blot analysis of extracts of rat thymus, using DNM1L antibody (144-02586) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - DNM1L Polyclonal Antibody (144-02586)

Immunohistochemistry of paraffin-embedded human liver injury using DNM1L antibody (144-02586) at dilution of 1:100 (40x lens).
Immunohistochemistry - DNM1L Polyclonal Antibody (144-02586)

Immunofluorescence analysis of HeLa cells using DNM1L antibody (144-02586). Blue: DAPI for nuclear staining.
Immunofluorescence - DNM1L Polyclonal Antibody (144-02586)

Expiration:

12 months from the date of shipment when stored properly.

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