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Anti-DNMT3B Antibody
Catalog #: 144-02899
DNMT3B Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9UBC3 |
| Gene Id | 1789 |
| Gene Symbols | DNMT3B |
| Protein Name / Synonyms | DNMT3B, ICF, ICF1, M.HsaIIIB, DNA methyltransferase 3 beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
Dilutions
WB 1:200 - 1:1000
Format
Calculated MW
77-95kDa
Observed MW
110kDa
Immunogen
A synthetic peptide of human DNMT3B
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DNMT3B antibody (144-02899) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.
Expiration:
12 months from the date of shipment when stored properly.