RayBiotech

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ISO 13485:2016 cGMP

Anti-DOK4 Antibody

Catalog #: 144-00226

DOK4 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q8TEW6
Gene Id
55715
Gene Symbols
DOK4
Protein Name / Synonyms
DOK4, IRS-5, IRS5, docking protein 4
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
37kDa
Observed MW
37kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 240-326 of human DOK4 (NP_060580.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of MCF-7 cells, using DOK4 antibody (144-00226) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - DOK4 Polyclonal Antibody (144-00226)

Immunofluorescence analysis of HeLa cells using DOK4 antibody (144-00226). Blue: DAPI for nuclear staining.
Immunofluorescence - DOK4 Polyclonal Antibody (144-00226)

Expiration:
12 months from the date of shipment when stored properly.