Anti-DPP3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9NY33
Gene Id	10072
Gene Symbols	DPP3
Protein Name / Synonyms	DPP3, DPPIII, dipeptidyl peptidase 3
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein that is a member of the M49 family of metallopeptidases. This cytoplasmic protein binds a single zinc ion with its zinc-binding motif (HELLGH) and has post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Increased activity of this protein is associated with endometrial and ovarian cancers. Alternatively spliced transcript variants have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

35kDa/69kDa/79kDa/82kDa

Observed MW

88kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human DPP3 (NP_569710.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using DPP3 antibody (144-60318) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - DPP3 antibody (144-60318)

Immunohistochemistry of paraffin-embedded human liver injury using DPP3 antibody (144-60318) at dilution of 1:100 (40x lens).
Immunohistochemistry - DPP3 antibody (144-60318)

Immunohistochemistry of paraffin-embedded human gastric cancer using DPP3 antibody (144-60318) at dilution of 1:100 (40x lens).
Immunohistochemistry - DPP3 antibody (144-60318)

Expiration:

12 months from the date of shipment when stored properly.

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