RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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ISO 13485:2016 cGMP

Anti-DPP8 Antibody

Catalog #: 144-07113

DPP8 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q6V1X1
Gene Id
54878
Gene Symbols
DPP8
Protein Name / Synonyms
DPP8, DP8, DPRP-1, DPRP1, MST097, MSTP097, MSTP135, MSTP141, dipeptidyl peptidase 8
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the peptidase S9B family, a small family of dipeptidyl peptidases that are able to cleave peptide substrates at a prolyl bond. The encoded protein shares similarity with dipeptidyl peptidase IV in that it is ubiquitously expressed, and hydrolyzes the same substrates. These similarities suggest that, like dipeptidyl peptidase IV, this protein may play a role in T-cell activation and immune function. Alternatively spliced transcript variants encoding different isoforms have been described.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW
83kDa/89kDa/97kDa/101kDa/103kDa
Observed MW
103kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 10-230 of human DPP8 (NP_932065.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using DPP8 antibody (144-07113) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - DPP8 antibody (144-07113)

Immunofluorescence analysis of A549 cells using DPP8 antibody (144-07113). Blue: DAPI for nuclear staining.
Immunofluorescence - DPP8 antibody (144-07113)

Expiration:
12 months from the date of shipment when stored properly.