Anti-DUT (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P33316
Gene Id	1854
Gene Symbols	DUT
Protein Name / Synonyms	DUT (C-term)
Clonality	Polyclonal
Preparation	This DUT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-198 amino acids from the C-terminal region of human DUT.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Autoimmune Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

Specificity

Predicted Reactivity

Rat

Other Names

DUT; Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; dUTP pyrophosphatase

NCBI Accession #

NP_001020419.1;NP_001020420.1;NP_001939.1

Other Accession #

P70583; NP_001939.1; NP_001020419.1; NP_001020420.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

26563

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of lysate from A431 cell line, using DUT Antibody (C-term)(Cat# 102-12362). 102-12362 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
	DUT Antibody (C-term) (102-12362)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DUT Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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