Anti-E2F1 (S332) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q01094
Gene Id	1869
Gene Symbols	E2F1
Protein Name / Synonyms	E2F1 (S332)
Clonality	Polyclonal
Preparation	This E2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310-344 amino acids from human E2F1.
Concentration (lot specific)	0.400 mg/ml
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another two members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Specificity

Other Names

E2F1; RBBP3; Transcription factor E2F1; PBR3; PRB-binding protein E2F-1; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3

NCBI Accession #

NP_005216.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

46920

Recommended Dilutions

WB: 1:1000

Western blot analysis of anti-E2F1 Antibody (S332) (Cat# 102-17439) in 293 cell line lysates (35ug/lane). E2F1 (arrow) was detected using the purified Pab.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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