3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-EDA Antibody
Catalog #: 144-02905
EDA Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q92838 |
| Gene Id | 1896 |
| Gene Symbols | EDA |
| Protein Name / Synonyms | EDA, ECTD1, ED1, ED1-A1, ED1-A2, EDA-A1, EDA-A2, EDA1, EDA2, HED, HED1, ODT1, STHAGX1, TNLG7C, XHED, XLHED, ectodysplasin-A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
14kDa/15kDa/40kDa/41kDa
Observed MW
48kDa
Immunogen
A synthetic peptide of human EDA
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EDA antibody (144-02905) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human uterine cancer using EDA Antibody (144-02905) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using EDA Antibody (144-02905) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using EDA Antibody (144-02905) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using EDA Antibody (144-02905) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse skeletal muscle using EDA Antibody (144-02905) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using EDA Antibody (144-02905) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.