Anti-EDA (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q92838
Gene Id	1896
Gene Symbols	EDA
Protein Name / Synonyms	EDA (N-term)
Clonality	Polyclonal
Preparation	This EDA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-76 amino acids from the N-terminal region of human EDA.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

EDA is a type II membrane protein that can be cleaved by furin to produce a secreted form. This protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in the gene for EDA are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia.

Specificity

Predicted Reactivity

Bovine

Other Names

EDA; ED1; EDA2; Ectodysplasin-A; Ectodermal dysplasia protein; Ectodysplasin-A, membrane form; Ectodysplasin-A, secreted form

NCBI Accession #

NP_001005609.1;NP_001005610.2;NP_001005612.2;NP_001005613.1;NP_001390.1

Other Accession #

Q9BEG5

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

41294

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of lysate from RD cell line,using EDA Antibody (N-term)(Cat# 102-16721).102-16721 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EDA antibody (N-term) (Cat# 102-16721), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of hela cells using EDA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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