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Anti-EDF1 Antibody
Catalog #: 144-02283
EDF1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O60869 |
| Gene Id | 8721 |
| Gene Symbols | EDF1 |
| Protein Name / Synonyms | EDF1, CFAP280, EDF-1, MBF1, endothelial differentiation-related factor 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a protein that may regulate endothelial cell differentiation, lipid metabolism, and hormone-induced cardiomyocyte hypertrophy. The encoded protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Alternate splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
15kDa/16kDa
Observed MW
16kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human EDF1 (NP_003783.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EDF1 antibody (144-02283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using EDF1 antibody (144-02283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using EDF1 antibody (144-02283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EDF1 antibody (144-02283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using EDF1 antibody (144-02283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using EDF1 antibody (144-02283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using EDF1 antibody (144-02283) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.