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Anti-EDN1 Antibody
Catalog #: 144-00686
EDN1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P05305 |
| Gene Id | 1906 |
| Gene Symbols | EDN1 |
| Protein Name / Synonyms | EDN1, ARCND3, ET1, HDLCQ7, PPET1, QME, endothelin-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cardiovascular Disease, Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
24kDa
Observed MW
24kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 18-212 of human EDN1 (NP_001946.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EDN1 antibody (144-00686) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human lung cancer using EDN1 antibody (144-00686) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using EDN1 antibody (144-00686) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.