Anti-EDNRB (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P24530
Gene Id	1910
Gene Symbols	EDNRB
Protein Name / Synonyms	EDNRB (C-term)
Clonality	Polyclonal
Preparation	This EDN_ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 409-439 amino acids from the C-terminal region of human EDN_.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

EDNRB is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene.

Specificity

Predicted Reactivity

Rat, Bovine, Pig, Rabbit

Other Names

EDNRB; ETRB; Endothelin B receptor; Endothelin receptor non-selective type

NCBI Accession #

NP_000106.1;NP_001116131.1;NP_001188326.1;NP_003982.1

Other Accession #

P21451; Q9N0W7; P35463; P48302; P28088

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

49644

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of EDNRB Antibody (C-term) (Cat# 102-16939) in mouse cerebellum tissue lysates (35ug/lane). EDNRB (arrow) was detected using the purified Pab.
	EDNRB Antibody (C-term) (Cat# 102-16939)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EDNRB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of EDNRB Antibody (C-term)(Cat# 102-16939) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	EDNRB Antibody (C-term) (Cat# 102-16939) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products