Anti-EEF1B2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P24534
Gene Id	1933
Gene Symbols	EEF1B2
Protein Name / Synonyms	EEF1B2, EEF1B, EEF1B1, EF1B, elongation factor 1-beta
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

24kDa

Observed MW

33kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human EEF1B2 (NP_001032752.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using EEF1B2 antibody (144-06580) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] EEF1B2 Polyclonal Antibody (144-06580)

Western blot analysis of extracts from normal (control) and EEF1B2 knockout (KO) 293T cells, using EEF1B2 antibody (144-06580) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - [KO Validated] EEF1B2 Polyclonal Antibody (144-06580)

Immunohistochemistry of paraffin-embedded mouse brain using EEF1B2 Antibody (144-06580) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (144-06580)

Immunohistochemistry of paraffin-embedded rat spinal cord using EEF1B2 Antibody (144-06580) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (144-06580)

Immunohistochemistry of paraffin-embedded rat brain using EEF1B2 Antibody (144-06580) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (144-06580)