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Anti-EEF1G Antibody
Catalog #: 144-07891
EEF1G Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P26641 |
| Gene Id | 1937 |
| Gene Symbols | EEF1G |
| Protein Name / Synonyms | EEF1G, EF1G, GIG35, elongation factor 1-gamma |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
50kDa/56kDa
Observed MW
50kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 168-437 of human EEF1G (NP_001395.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EEF1G antibody (144-07891) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using EEF1G antibody (144-07891) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using EEF1G antibody (144-07891) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.